1VP8

CRYSTAL STRUCTURE OF an alpha/beta domain of a putative pyruvate kinase (AF0103) FROM ARCHAEOGLOBUS FULGIDUS DSM 4304 AT 1.30 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-28
Detector	ADSC
Spacegroup name	P 63 2 2
Unit cell lengths	109.650, 109.650, 76.451
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.610 - 1.300
R-factor	0.15499
Rwork	0.154
R-free	0.16633
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.620
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.900	1.330
High resolution limit [Å]	1.300	1.300
Number of reflections	66691
<I/σ(I)>	21.9	2
Completeness [%]	100.0	100
Redundancy	14.4	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	50.0% PEG-200, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	50.0% PEG-200, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	50.0% PEG-200, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K