1VP7

Crystal structure of Exodeoxyribonuclease VII small subunit (NP_881400.1) from Bordetella pertussis at 2.40 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-18
Detector	ADSC
Spacegroup name	P 6 2 2
Unit cell lengths	107.030, 107.030, 207.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.020 - 2.400
R-factor	0.20473
Rwork	0.203
R-free	0.24440
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.605
Data reduction software	MOSFLM
Data scaling software	SCALA (5.0))
Phasing software	autoSHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	28264
<I/σ(I)>	18	3.6
Completeness [%]	99.9	100
Redundancy	11.7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	2.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	2.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	2.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K