1VM8

Crystal structure of UDP-N-acetylglucosamine pyrophosphorylase (Agx2) from Mus musculus at 2.50 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-05
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	80.094, 73.402, 107.132
Unit cell angles	90.00, 99.83, 90.00

Refinement procedure

Resolution	29.110 - 2.500
R-factor	0.20971
Rwork	0.207
R-free	0.26573
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.417
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.110	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	42134
<I/σ(I)>	9.5	2.7
Completeness [%]	99.3	96.9
Redundancy	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	1.6M (NH4)2SO4, 0.1M Cacodylate pH 6.5, 0.2M NaCl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	1.6M (NH4)2SO4, 0.1M Cacodylate pH 6.5, 0.2M NaCl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	1.6M (NH4)2SO4, 0.1M Cacodylate pH 6.5, 0.2M NaCl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K