1VLP

Crystal structure of a putative nicotinate phosphoribosyltransferase (yor209c, npt1) from saccharomyces cerevisiae at 1.75 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-08
Detector	ADSC
Spacegroup name	P 1
Unit cell lengths	54.406, 83.103, 107.237
Unit cell angles	97.35, 95.67, 97.99

Refinement procedure

Resolution	48.240 - 1.750
R-factor	0.17163
Rwork	0.170
R-free	0.20962
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.544
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXCD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.075	0.451
Number of reflections	177740
<I/σ(I)>	18.09	1.64
Completeness [%]	96.8	90.05
Redundancy	3.79	2.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.06M MES, 0.04M MES_Na, 14% PEG MME 5000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.06M MES, 0.04M MES_Na, 14% PEG MME 5000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	0.06M MES, 0.04M MES_Na, 14% PEG MME 5000 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K