1VKD

Crystal structure of a predicted glycosidase (tm1225) from thermotoga maritima msb8 at 2.10 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-04
Detector	ADSC
Wavelength(s)	1.115896, 0.979508, 0.979740, 1.019778
Spacegroup name	P 21 21 21
Unit cell lengths	86.867, 100.908, 253.433
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.170 - 2.100
R-factor	0.16417
Rwork	0.162
R-free	0.20404
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.418
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.910	2.150
High resolution limit [Å]	2.098	2.100
Number of reflections	119326
<I/σ(I)>	11.3	1.5
Completeness [%]	91.2	78.4
Redundancy	4.1	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	293	Cacodylate pH 6.5, 5% PEG-1000, 0.2M MgCl2 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K