1VK5
X-ray Structure of Gene Product from Arabidopsis Thaliana At3g22680
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 83.450, 83.450, 60.575 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.300 - 1.604 |
R-factor | 0.16102 |
Rwork | 0.160 |
R-free | 0.18350 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.646 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.05) |
Refinement software | REFMAC (refmac_5.1.24) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.037 | 0.021 | 0.302 |
Number of reflections | 32231 | ||
<I/σ(I)> | 38.61 | 9.3 | |
Completeness [%] | 99.2 | 99.8 | 92.2 |
Redundancy | 10.9 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | protein (10 mg/ml), Ammonium Sulfate (0.8M), Hepes (0.1M), Magnesium Sulfate (0.04M), CHAPS (0.41%), pH 8.5, Vapor diffusion, hanging drop, temperature 293K |
2 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | protein (5 mg/ml), Ammonium Sulfate (0.8M), Hepes (0.1M), Magnesium Sulfate (0.04M), CHAPS (0.41%), pH 8.5, Vapor diffusion, hanging drop, temperature 293K |