1VK0
X-ray Structure of Gene Product from Arabidopsis Thaliana At5g06450
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9792, 0.9800, 0.9641 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 120.831, 120.831, 185.222 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.922 - 2.100 |
R-factor | 0.1829 |
Rwork | 0.180 |
R-free | 0.23260 |
Structure solution method | MAD |
RMSD bond length | 0.023 |
RMSD bond angle | 1.777 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.02) |
Refinement software | REFMAC (refmac_5.1.24) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.690 | 2.100 |
Rmerge | 0.062 | 0.031 | 0.479 |
Number of reflections | 91738 | ||
<I/σ(I)> | 29 | 5.8 | |
Completeness [%] | 100.0 | 100 | 99.8 |
Redundancy | 10.6 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Sodium Citrate, Polyethylene glycol 2000, hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |