1VJO
Crystal structure of Alanine--glyoxylate aminotransferase (ALR1004) from Nostoc sp. at 1.70 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-29 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.258, 101.057, 97.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.180 - 1.700 |
| R-factor | 0.15383 |
| Rwork | 0.151 |
| R-free | 0.20200 * |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.590 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA (4.2)) |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.180 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 * | 0.431 * |
| Total number of observations | 313619 * | |
| Number of reflections | 39819 * | |
| <I/σ(I)> | 11.8 | 2 |
| Completeness [%] | 92.0 * | 74.9 * |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 * | 293 | 0.06M HEPES, 0.04M HEPES_Na, 12% PEG MME 5000, 0.2M NP_Calcium Chloride , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 12 (%) | |
| 2 | 1 | reservoir | 0.2 (M) | pH7.5 | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | |
| 4 | 1 | reservoir | ethylene glycol | 10 (%) | |
| 5 | 1 | drop | Tris | 10 (mM) | pH7.8 |
| 6 | 1 | drop | 150 (mM) | ||
| 7 | 1 | drop | protein | 22 (mg/ml) |
