1VJK
Putative molybdopterin converting factor, subunit 1 from Pyrococcus furiosus, Pfu-562899-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 81.467, 81.467, 63.698 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.711 - 1.510 |
| Rwork | 0.210 |
| R-free | 0.22430 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.322 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC (refmac_5.1.24) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.560 |
| High resolution limit [Å] | 1.510 | 3.250 | 1.510 |
| Rmerge | 0.065 | 0.058 | 0.160 |
| Number of reflections | 19993 | ||
| Completeness [%] | 99.3 | 93.3 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 6.5 | 291 | 100mM sodium cacodylate, 30% PEG 8000, 200mM ammonium sulfate, modified microbatch, temperature 291K, pH 6.5 |
