1VJI

Gene Product of At1g76680 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Detector technology	CCD
Collection date	2003-10-26
Detector	MARRESEARCH
Wavelength(s)	0.96400
Spacegroup name	C 2 2 21
Unit cell lengths	46.688, 88.066, 149.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.850 - 2.000 *
Rwork	0.206
R-free	0.29300
RMSD bond length	0.024
RMSD bond angle	1.890 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE (CCP4 4.2)
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.850 *	50.000	2.050
High resolution limit [Å]	2.000	4.930	2.000
Rmerge	0.065 *	0.025	0.816 *
Number of reflections	20780 *
Completeness [%]	98.2	99.9	92
Redundancy	3.6 *		3.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	277	Methyl ether, Polyethylene glycol 5k,Calcium Chloride, MES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	10 (mM)	pH8.0
3	1	drop		100 (mM)
4	1	drop	TCEP	0.3 (mM)
5	1	reservoir	PEG5000 MME	8.75-10 (%)
6	1	reservoir	TEA	0.1 (M)	pH8.0
7	1	reservoir	glycine	0.275-0.35 (M)