1VGP
Crystal Structure of an Isozyme of Citrate Synthase from Sulfolbus tokodaii strain7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.600, 121.600, 108.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.990 - 2.700 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iom |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.233 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.097 | 0.470 |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 99.3 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Formate, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
