1VG1
GDP-Bound Rab7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-02 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.500, 103.200, 51.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.790 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rab7 from REP1:Rab7 complex (PDB code 1VG0) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.800 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 13877 | |
| <I/σ(I)> | 20.2 | 5.7 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 25% PEG 3350, 0.25M Ammonium Phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
