1VFY

PHOSPHATIDYLINOSITOL-3-PHOSPHATE BINDING FYVE DOMAIN OF VPS27P PROTEIN FROM SACCHAROMYCES CEREVISIAE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	95
Detector technology	CCD
Collection date	1999-02-15
Detector	ADSC
Wavelength(s)	1.2830,1.2822,1.2320
Spacegroup name	P 1
Unit cell lengths	24.090, 26.570, 31.610
Unit cell angles	111.79, 92.70, 105.70

Refinement procedure

Resolution	30.000 - 1.150
Rwork	0.174
R-free	0.18100
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.770
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	1.150	2.000
Rmerge	0.065	0.011
Number of reflections	20972
<I/σ(I)>	18.6	8
Completeness [%]	95.4	82.3
Redundancy	3.8	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *	0.2 M AMMONIUM ACETATE, 0.1 M SODIUM ACETATE PH 4.6, 15% PEG 4000, pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13-15 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	dithiothreitol	10 (mM)
4	1	reservoir	ammonium acetate	0.2 (M)
5	1	reservoir	sodium acetate	0.1 (M)
6	1	reservoir	PEG4000	15 (%)