1VFG

Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-12-09
Detector	RIGAKU RAXIS V
Wavelength(s)	1.000, 0.9793, 0.9795, 0.9843, 0.9743
Spacegroup name	P 1 2 1
Unit cell lengths	109.476, 125.900, 58.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.800
Rwork	0.230
R-free	0.28650
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.520
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	39382
<I/σ(I)>	22.4	4.3
Completeness [%]	99.2	95.7
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	MPD, magnesium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
2	1	1	magnesium acetate
3	1	2	MPD
4	1	2	magnesium acetate