1VFG
Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-09 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.000, 0.9793, 0.9795, 0.9843, 0.9743 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 109.476, 125.900, 58.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.800 |
| Rwork | 0.230 |
| R-free | 0.28650 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.520 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 39382 | |
| <I/σ(I)> | 22.4 | 4.3 |
| Completeness [%] | 99.2 | 95.7 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MPD, magnesium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 2 | MPD | ||
| 4 | 1 | 2 | magnesium acetate |
