1VF8
The Crystal Structure of Ym1 at 1.31 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.611, 60.123, 60.106 |
| Unit cell angles | 90.00, 94.67, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.310 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e9l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.360 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.068 | 0.253 |
| Number of reflections | 79157 | |
| <I/σ(I)> | 13.4 | 3.2 |
| Completeness [%] | 91.7 | 93.7 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7 | 277 | distilled water, pH 7.0, MICRODIALYSIS, temperature 277K |
