1VEW
MANGANESE SUPEROXIDE DISMUTASE FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-C |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.840, 108.910, 182.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.186 |
| Rwork | 0.188 |
| R-free | 0.21800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mng |
| RMSD bond length | 0.010 |
| RMSD bond angle | 15.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.056 * | 0.191 * |
| Total number of observations | 215051 * | |
| Number of reflections | 56890 | |
| <I/σ(I)> | 22 | 5.4 |
| Completeness [%] | 96.8 | 77 |
| Redundancy | 3.7 | 0.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | CRYSTALS WERE GROWN AT ROOM TEMPERATURE USING THE HANGING DROP METHOD. THE HANGING DROPS WERE SUSPENDED OVER 0.75ML OF WELL SOLUTION (16-30% PEG6000 AND 0.05M BICINE TITRATED TO PH8.5) AND CONSISTED OF 2UL OF WELL SOLUTION AND 2UL OF PROTEIN SOLUTION (MNSOD AT 12MG/ML IN WATER)., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 8-15 (%) | |
| 3 | 1 | drop | bicine titrated | 0.025 (M) | |
| 4 | 1 | reservoir | PEG6000 | 16-30 (%) | |
| 5 | 1 | reservoir | bicine titrated | 0.05 (M) |
