1VD5
Crystal Structure of Unsaturated Glucuronyl Hydrolase, Responsible for the Degradation of Glycosaminoglycan, from Bacillus sp. GL1 at 1.8 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.72 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 103.012, 103.012, 223.039 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.800 |
Rwork | 0.168 |
R-free | 0.18900 |
Structure solution method | MIR |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 63892 | |
Completeness [%] | 96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | MPD, sodium chloride, glycine, DTT, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |