1VCY
VVA2 isoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 133 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 56.107, 56.107, 132.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.510 - 2.600 |
| Rwork | 0.225 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pp0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.510 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.072 | |
| Number of reflections | 7022 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | ammonium sulfate, PEGMME 5000, malonic acid, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
