1VAL
CONCANAVALIN A COMPLEX WITH 4'-NITROPHENYL-ALPHA-D-GLUCOPYRANOSIDE
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-10-20 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 134.660, 155.670, 71.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 3.000 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.27400 |
RMSD bond length | 0.006 |
RMSD bond angle | 26.800 * |
Data reduction software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | |
High resolution limit [Å] | 3.000 | 3.000 * |
Rmerge | 0.143 | 0.439 * |
Total number of observations | 116891 * | |
Number of reflections | 30575 | |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 10 (mM) | |
2 | 1 | reservoir | ammonium sulfate | 1 (M) | |
3 | 1 | drop | ConA | 70 (mg/ml) | |
4 | 1 | drop | saccharide | 10 * molar excess |