1V9Y
Crystal Structure of the heme PAS sensor domain of Ec DOS (ferric form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.71 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.740, 69.780, 82.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.320 |
R-factor | 0.16 |
Rwork | 0.160 |
R-free | 0.21930 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 0.030 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.390 |
High resolution limit [Å] | 1.320 | 1.320 |
Number of reflections | 60488 | |
<I/σ(I)> | 23.9 | 1.9 |
Completeness [%] | 98.6 | 100 |
Redundancy | 5.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | ammonium sulfate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |