1V9M
Crystal structure of the C subunit of V-type ATPase from Thermus thermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-02 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.9793, 0.9795, 0.9781 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.790, 83.806, 48.567 |
Unit cell angles | 90.00, 113.86, 90.00 |
Refinement procedure
Resolution | 19.620 - 1.850 |
Rwork | 0.202 |
R-free | 0.22800 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.061 | 0.338 |
Number of reflections | 25341 | |
<I/σ(I)> | 20 | 3.7 |
Completeness [%] | 97.1 | 91.5 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 300, PEG 8000, glycerol, Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |