1V9I
Crystal Structure Analysis of the site specific mutant (Q253C) of bovine carbonic anhydrase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.978 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 73.100, 73.100, 116.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.950 |
Rwork | 0.247 |
R-free | 0.34600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.950 |
Number of reflections | 7798 |
Completeness [%] | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 7.5 | 277 | 50mM Tris-HCl, 3mM NaN3, 2.3M Ammonium Sulfate, pH 7.5, SMALL TUBES, temperature 277K |