1V9F
Crystal structure of catalytic domain of pseudouridine synthase RluD from Escherichia coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9000, 0.9788, 0.9795, 0.9814 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 74.506, 74.506, 265.009 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.490 - 1.700 |
R-factor | 0.20094 |
Rwork | 0.200 |
R-free | 0.22420 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.452 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.058 | 0.241 |
Number of reflections | 47799 | |
<I/σ(I)> | 18.1 | |
Completeness [%] | 98.7 | 91 |
Redundancy | 15.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ethylene glycol, PEG3000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |