1V96
Crystal structure of hypothetical protein of unknown function from pyrococcus horikoshii OT3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-23 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.9792, 0.9795, 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.909, 95.463, 102.656 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.22800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 2.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.039 | 0.199 |
Number of reflections | 45126 | |
Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 6.4% PEG 4000, 0.1M CITRATE, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |