1V7Y
Crystal structure of tryptophan synthase alpha-subunit from Escherichia coli at room temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1994-07-01 |
Detector | MAC Science DIP-2000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 158.420, 45.100, 72.060 |
Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
Resolution | 19.560 - 2.500 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.560 | 2.660 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 15954 | |
Completeness [%] | 89.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | Ammonium sulfate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |