1V7V
Crystal structure of Vibrio proteolyticus chitobiose phosphorylase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-23 |
| Detector | ACSC QUANTUM 210 |
| Wavelength(s) | 1.0000, 0.97120, 0.97848, 0.97960 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.946, 70.580, 80.045 |
| Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
| Resolution | 47.780 - 1.800 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.18600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.049 | 0.228 |
| Number of reflections | 70781 | |
| <I/σ(I)> | 15.3 | 5.33 |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG400, HEPES, Calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
