1V7U
Crystal structure of Undecaprenyl Pyrophosphate Synthase with farnesyl pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.030, 69.200, 108.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.350 |
| R-factor | 0.254 |
| Rwork | 0.173 |
| R-free | 0.25400 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.870 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.129 | 0.363 * |
| Total number of observations | 95659 * | |
| Number of reflections | 19508 | 1882 * |
| <I/σ(I)> | 11 | 11 |
| Completeness [%] | 97.1 | 95.1 * |
| Redundancy | 4.65 | 4.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 25 * | 25% ethylene glycol, 0.05% triton, 0.5mM MgCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ethylene glycol | 25 (%) | pH7.5 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | drop | peptide | 1 (mM) | |
| 4 | 1 | drop | Triton X-100 | 0.05 (%) | |
| 5 | 1 | drop | 0.5 (mM) |
