1V7M
Human Thrombopoietin Functional Domain Complexed To Neutralizing Antibody TN1 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-12-07 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.099, 46.771, 185.151 |
Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
Resolution | 15.000 * - 2.500* |
R-factor | 0.23185 |
Rwork | 0.228 |
R-free | 0.31500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iai |
RMSD bond length | 0.020 |
RMSD bond angle | 2.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.074 * | 0.318 * |
Total number of observations | 120117 * | |
Number of reflections | 36766 | |
<I/σ(I)> | 8.1 | 1.1 |
Completeness [%] | 92.0 | 71 |
Redundancy | 2.6 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 295 * | Kuroki, R., (2002) Acta Crystallogr.,Sect.D, 58, 856. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18-20 (mg/ml) | |
2 | 1 | drop | imidazole | 20 (mM) | pH7.0 |
3 | 1 | reservoir | PEG3350 | 19-21 (%(w/v)) | |
4 | 1 | reservoir | potassium phosphate | 100 (mM) | pH6.0 |