1V7L
Structure of 3-isopropylmalate isomerase small subunit from Pyrococcus horikoshii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979131, 0.979359, 0.97001 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 95.208, 95.208, 152.258 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.980 |
Rwork | 0.206 |
R-free | 0.24700 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.640 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.090 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.084 | 0.389 |
Number of reflections | 29033 | |
<I/σ(I)> | 7.1 | 2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 15.1 | 15.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 0.1M HEPES, 1.4M AMMONIUM SULFATE, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |