1V6T
Crystal Structure of Lactam Utilization Protein from Pyrococcus horikoshii Ot3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-18 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.00, 0.97914, 0.97964 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.366, 74.288, 105.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.780 - 1.700 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.259 |
| Number of reflections | 28634 | |
| <I/σ(I)> | 20.1 | 6.35 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 5.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.2 | 295 | sodium chloride, acetate, pH 4.2, microbatch, temperature 295K |
