1V5H
Crystal Structure of Human Cytoglobin (Ferric Form)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0, 1.7409, 1.7394, 1.7364, 1.7432 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.401, 74.401, 59.634 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 7348 | |
| Completeness [%] | 96.1 | 76.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.2 | 293 | Sodium Citrate, CHES, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
