1V4S

Crystal structure of human glucokinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	2001-07-04
Detector	WEISSENBERG
Wavelength(s)	1.000
Spacegroup name	P 65 2 2
Unit cell lengths	79.855, 79.855, 322.221
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.420 - 2.300
R-factor	0.235
Rwork	0.232
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cza
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNX (2002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.420	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	27833
<I/σ(I)>	22.1	4.2
Completeness [%]	98.5	96.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	PEG1500, Hepes, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K