1V3M
Crystal structure of F283Y mutant cyclodextrin glycosyltransferase complexed with a pseudo-tetraose derived from acarbose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2001-06-18 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 64.090, 73.600, 78.300 |
Unit cell angles | 84.93, 105.12, 101.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.167 |
Rwork | 0.163 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pam |
RMSD bond length | 0.013 |
RMSD bond angle | 2.760 |
Data reduction software | FFFEAR |
Data scaling software | FFFEAR |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.900 | 1.880 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.095 | |
Number of reflections | 103110 | |
Redundancy | 2.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG3000, SODIUM CITRATE, 2-PROPANOL, CALCIUM CHLORIDE, ACARBOSE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |