1V0A

Family 11 Carbohydrate-Binding Module of cellulosomal cellulase Lic26A-Cel5E of Clostridium thermocellum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	110
Wavelength(s)	0.981033,0.981266, 0.976315
Spacegroup name	P 21 21 2
Unit cell lengths	75.143, 50.892, 40.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 - 1.980
R-factor	0.195
Rwork	0.193
R-free	0.23200
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.829
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.299	2.020
High resolution limit [Å]	1.977	1.980
Rmerge	0.059	0.099
Number of reflections	11449
<I/σ(I)>	30.3	8.4
Completeness [%]	99.5	99.3
Redundancy	1.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.00