1UZZ

Erythrina cristagalli bound to N-linked oligosaccharide and lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	293
Detector technology	CCD
Collection date	2002-02-15
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	55.280, 55.370, 86.930
Unit cell angles	86.23, 75.37, 82.13

Refinement procedure

Resolution	40.000 - 2.130
R-factor	0.191
Rwork	0.191
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.210
High resolution limit [Å]	2.130	2.130
Rmerge	0.070	0.200
Number of reflections	55200
<I/σ(I)>	10.9	5.2
Completeness [%]	97.6	86.1
Redundancy	1.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		17% PEG 3350, 0.3M SODIUM CHLORIDE,0.02M IMIDAZOLE, pH 7.00