1UZX

A complex of the Vps23 UEV with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	58.934, 66.032, 69.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.690 - 1.850
R-factor	0.241
Rwork	0.241
R-free	0.27400
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.066	0.320
Number of reflections	23553
<I/σ(I)>	22.7	2.36
Completeness [%]	99.3	97.2
Redundancy	9.07	3.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		22% PEG 8000, 0.1M MES PH 6.5, 0.2M (NH4)2SO4