1UZM
MabA from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.274, 116.991, 51.977 |
| Unit cell angles | 90.00, 122.05, 90.00 |
Refinement procedure
| Resolution | 59.000 - 1.490 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.244 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.000 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.026 | 0.411 |
| Number of reflections | 60917 | |
| <I/σ(I)> | 0.9 | |
| Completeness [%] | 92.9 | 70.9 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | PIPES PH 6.6, CSCL 300 MM, PEG 3000 12% |
