1UZJ

Integrin binding cbEGF22-TB4-cbEGF33 fragment of human fibrillin-1, holo form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-15
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	43.100, 105.600, 65.000
Unit cell angles	90.00, 104.20, 90.00

Refinement procedure

Resolution	30.000 - 2.250
R-factor	0.247
Rwork	0.247
R-free	0.31800
Structure solution method	MAD
Starting model (for MR)	PBD FIBRILLIN I222 FORM
RMSD bond length	0.009
RMSD bond angle	1.540
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.099	0.450
Number of reflections	25753
<I/σ(I)>	9.7	2.2
Completeness [%]	96.2	92.6
Redundancy	2.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		5MG/ML PROTEIN IN 10MM MES 10MM CACL2 PH6. DEHYDRATED AGAINST: 15% PEG 8000, 10MM MES PH 6 10MM CACL2