1UZB

1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-02-15
Detector	RIGAKU RAXIS-V
Spacegroup name	H 3
Unit cell lengths	102.285, 102.285, 279.283
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 1.400
R-factor	0.177
Rwork	0.177
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bi9
RMSD bond length	0.005
RMSD bond angle	22.700 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.045	0.181
Number of reflections	211697
<I/σ(I)>	38.99	7.75
Completeness [%]	98.6	97.1
Redundancy	3.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	298 *	Inagaki, E., (2005) Acta Cryst., F61, 609. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	MPD	32 (%)
3	1	reservoir	sodium citrate	50 (mM)	pH5.2