1UZ8
anti-Lewis X Fab fragment in complex with Lewis X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.104, 60.778, 91.556 |
| Unit cell angles | 95.98, 95.41, 101.71 |
Refinement procedure
| Resolution | 91.290 - 1.800 |
| Rwork | 0.210 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uz6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.151 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.175 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.094 | 0.386 |
| Number of reflections | 81582 | |
| <I/σ(I)> | 11.1 | 2.2 |
| Completeness [%] | 92.6 | 72 |
| Redundancy | 4.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 14 % PEG 3350, 50 MM TRIS PH 8 |
