1UZ6
anti-Lewis X Fab fragment uncomplexed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.405, 71.559, 104.790 |
| Unit cell angles | 86.48, 71.32, 83.26 |
Refinement procedure
| Resolution | 45.180 - 2.050 |
| Rwork | 0.198 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.144 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.175 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.046 | 0.329 |
| Number of reflections | 102864 | |
| <I/σ(I)> | 19.7 | 2.1 |
| Completeness [%] | 88.1 | 38.9 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 26 % PEG 3350, 0.17 M AMMONIUM SULFATE, 15 % GLYCEROL, pH 8.00 |
