1UZ1
Family 1 b-glucosidase from Thermotoga maritima in complex with isofagomine lactam
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-15 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.412, 94.327, 113.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.550 - 2.000 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1od0 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.303 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | 0.437 |
Number of reflections | 65765 | |
<I/σ(I)> | 24.3 | 2.34 |
Completeness [%] | 96.0 | 84.5 |
Redundancy | 4.95 | 2.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 10MG/ML PROTEIN IN 15% PEG 4000, 0.2 M CAAC, 0.1 M IMIDAZOLE PH7, pH 7.00 |