1UYJ
Clostridium perfringens epsilon toxin shows structural similarity with the pore forming toxin aerolysin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.77, 1.653, 0.885 |
| Spacegroup name | P 63 |
| Unit cell lengths | 125.962, 125.962, 121.862 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.23905 |
| Rwork | 0.236 |
| R-free | 0.29006 |
| Structure solution method | MAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.045 | 0.200 |
| Number of reflections | 33253 | |
| <I/σ(I)> | 10.0438 | 2.8 |
| Completeness [%] | 98.3 | 97.2 |
| Redundancy | 8.91 | 8.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 25% ETHYLENE GLYCOL, pH 7.00 |
