1UXZ

Carbohydrate binding module (CBM6cm-2) from Cellvibrio mixtus lichenase 5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Collection date	2002-06-15
Spacegroup name	P 21 21 21
Unit cell lengths	63.110, 66.956, 85.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.520 - 1.400
R-factor	0.162
Rwork	0.161
R-free	0.17700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nae
RMSD bond length	0.010
RMSD bond angle	1.323
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.055	0.189
Number of reflections	70856
<I/σ(I)>	9.1	3.2
Completeness [%]	98.8	97.7
Redundancy	4.9	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		11% PEG 6000, 2.0 M NACL, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG6000	12 (%(w/v))
3	1	reservoir		2.2 (M)