1UXU

Structural basis for allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) from Thermoproteus tenax

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-15
Detector	MAR scanner 345 mm plate
Spacegroup name	P 62 2 2
Unit cell lengths	185.290, 185.290, 131.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.250
R-factor	0.231
Rwork	0.231
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ky8
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.350
High resolution limit [Å]	2.250	2.250
Rmerge	0.049	0.370
Number of reflections	58447
<I/σ(I)>	28	5
Completeness [%]	92.0	94
Redundancy	13	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		2M SODIUM FORMATE PH 5.6, 0.1 M SODIUM ACETATE PH 5.6, 50 MM AMP, 1 MM GLYCERALDEHYDE 3-PHOSPHATE