1UX8

X-ray structure of truncated oxygen-avid haemoglobin from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Spacegroup name	P 41 21 2
Unit cell lengths	41.193, 41.193, 134.537
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.500 - 2.150
R-factor	0.208
Rwork	0.206
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ngk
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.150
Rmerge	0.098
Number of reflections	6975
Completeness [%]	98.6
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		2,0 M AMMONIUM SULFATE, 5% V/V ISOPROPANOL, PH=6.0, pH 6.00