1UX5

Crystal Structures of a Formin Homology-2 domain reveal a flexibly tethered dimer architecture

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Wavelength(s)	0.916, 0.936
Spacegroup name	P 61 2 2
Unit cell lengths	101.400, 101.400, 265.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.207
Rwork	0.207
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.210
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.087	0.458 *
Total number of observations	154481 *
Number of reflections	49995
<I/σ(I)>	12.9
Completeness [%]	94.5 *	90 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	HANGING DROP AGAINST A WELL CONTAINING 16% ETHYLENE GLYCOL, pH 7.50
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	HANGING DROP AGAINST A WELL CONTAINING 16% ETHYLENE GLYCOL, pH 7.50
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	HANGING DROP AGAINST A WELL CONTAINING 16% ETHYLENE GLYCOL, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	PCMBS	2.5 (mM)
3	1	reservoir	ethylene glycol	16 (%)