1UWQ
Structure of beta-glycosidase from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 168.026, 168.026, 94.605 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 141.420 - 2.020 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gow |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.285 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.069 | 0.461 |
| Number of reflections | 99428 | |
| <I/σ(I)> | 17.3 | 3.15 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 4.89 | 4.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 11-14% PEG 4K 0.1 M NA ACETATE, 0.2 M AMMONIUM ACETATE CRYO - 25% ETHYLENE GLYCOL, 10-13 MG/ML PROTEIN, pH 4.60 |
