1UWL
1.76A Structure of Urocanate Hydratase from Pseudomonas putida
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Collection date | 2003-02-15 |
| Wavelength(s) | 0.89,0.9824,0.9820,0.9103 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.430, 71.622, 129.120 |
| Unit cell angles | 90.00, 98.99, 90.00 |
Refinement procedure
| Resolution | 24.330 - 1.760 |
| R-factor | 0.16924 |
| Rwork | 0.168 |
| R-free | 0.18860 |
| Structure solution method | MAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.056 | 0.230 |
| Number of reflections | 47378 | |
| <I/σ(I)> | 14.4 | 5.1 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | pH 5.80 |
