1UWB
TYR 181 CYS HIV-1 RT/8-CL TIBO
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Collection date | 1996-03 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 225.700, 69.200, 104.900 |
Unit cell angles | 90.00, 106.60, 90.00 |
Refinement procedure
Resolution | 15.000 * - 3.200 |
R-factor | 0.274 |
Rwork | 0.274 |
R-free | 0.36000 |
RMSD bond length | 0.011 |
RMSD bond angle | 23.700 * |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 * |
High resolution limit [Å] | 3.200 * |
Rmerge | 0.105 |
Total number of observations | 61059 * |
Number of reflections | 23319 * |
Completeness [%] | 90.0 * |
Redundancy | 2.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * | Clark, A.D., (1995) Methods Enzymol., 262, 171. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 19-20 (mg/ml) | |
2 | 1 | drop | beta-OG | 0.1 (%) | |
3 | 1 | drop | DMSO | 1.6 (%) | |
4 | 1 | reservoir | Bis-Tris propane | 50 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
6 | 1 | reservoir | glycerol | 10 (%) |